The twins were born prematurely at Rome’s Alma Res fertility clinic, Rai News reported on Tuesday.
Despite their early arrival, the newborn baby girl and boy are said to be in good health.
Their 47-year-old mother had tried unsuccessfully for 15 years to become pregnant, before turning to an egg donor last year.
The fertility treatment was made possible from June last year, after the constitutional court ruled the ban on egg and sperm donors went against Italians’ right to have a family.
The decision marked the end of a ten-year battle against legislation introduced in 2004 to outlaw such donations, introduced during Silvio Berlusconi’s second term as prime minister.
At least three women fell pregnant in Rome last July through the newly-available fertility treatment.