The Pavarotti family are not the first to ask the Trump camp to stop using their music: others include rock legends Queen, the Rolling Stones, Adele, Aerosmith and Neil Young.
"We would like to recall that the values of brotherhood and solidarity which Luciano Pavarotti expressed throughout the course of his artistic career are entirely incompatible with the world view expressed by the candidate Donald Trump," the family said in a statement.
"Nessun Dorma", which became Pavarotti's signature aria and climaxes with the words "Vincero" ("I will win"), has been played often at Trump rallies.
Pavarotti, the son of a baker and cigar factory worker who became one of the most commercially successful tenors of all time, died in 2007 of pancreatic cancer. He was 71.