The theatre doors were swung open on Monday evening and the orchestra’s performance of the Funeral March (Adagio assai), from Beethoven's Eroica, aired in the Piazza della Scala.
Thousands gathered in silence to hear the performance, conducted by La Scala’s Musical Director Daniel Barenboim.
The tribute came a week after the death of 80-year-old Abbado, who was the opera house’s musical director from 1965 until 1986.
After leaving his native Milan, Abbado was musical director of the Staatsoper in Vienna between 1986 and 1991. He also worked at the Vienna Philharmonic and conducted the London Symphony Orchestra between 1979 and 1988.
Watch the video of La Scala Philharmonic Orchestra’s tribute to Abbado: