The Spallanzani Institute said on Twitter that the 50-year-old medic - who has not been named - was in a "good condition" and was "recovering in isolation".
The father of two was repatriated in November from Sierra Leone with a fever and given an experimental drug to try to combat the often-deadly virus.
The doctor - who became the first Italian to be infected with Ebola while volunteering for an Italian medical charity fighting the epidemic - can now breathe, walk and eat unassisted.
Ebola, a disease transmitted through the bodily fluids of infected people, can result in death from uncontrollable bleeding and organ failure.
The current Ebola epidemic in West Africa - the worst ever recorded - has so far killed around 7,350 people.