Putin and Italy's disgraced former prime minister, who the Russian strongman counts among his close friends, visited an Italian war cemetery near the Black Sea port of Sevastopol, a spokeswoman for Crimea's regional leader told AFP.
The cemetery became the final resting place for many Italian soldiers who perished in the 1853-1856 Crimean War which involved armed forces from the Italian kingdom of Sardinia.
Berlusconi, who has repeatedly praised Putin's leadership in the past, is in Russia on a private visit expected to last until Sunday.
Putin and Berlusconi are longstanding friends who vacationed together in the past.
During a visit by Putin to Rome this June, the media tycoon pledged his Forza Italia party would work to lift Western sanctions on Russia over the Ukraine crisis.