Sporting Locri, a futsal team which competes in Italy's top Serie A league, looked set to close last month after its president received anonymous messages threatening both his toddler and the club.
Futsal is the increasingly popular brand of five-a-side indoor football.
Club President Ferdinando Armeni bowed to pressure and resigned before Christmas, but the capitulation sparked a national outcry, with the Italian Football Federation (FIGC) stepping in to insist the team must play on.
With the club now headed up temporarily by the town's mayor Giovanni Calabrese, Sporting Locri will square up to Lazio at 1700 GMT in a match broadcast live on national television and attended by FIGC president Carlo Tavecchio.
The Lazio team looked defiant Sunday as it flew in from Rome to Calabria, a region in the grip of the richest and most powerful syndicate in Europe and which in 2014 had the highest level of unemployment in Italy, at 23.4 percent.
Italy has launched a probe into the messages ordering Armeni to shut down the club, including a note left on the car-seat of his three-year-old daughter.
The former president, whose tyres were also slashed, told journalists he had no idea who was behind the threats — and brushed off press rumours that the real reason he resigned was because the club had finance problems after over-investing in players from Spain.
Top anti-mafia prosecutor Nicola Gratteri, who has lived under armed guard for the past 26 years after death threats and several attempts on his life, told AFP “the team absolutely must not close its doors”.
“They not only have to keep playing but they have to be given psychological assistance because I can only imagine the stress these poor girls have been put under,” he said, adding that the team was “a source of pride for this region”.
Football is a honeypot for the mafia, which makes vast profits from match-fixing as well as using the sport as a means to recycle ill-gotten gains.