The strike was called by workers' union Osr Sul to demand better pay and improved safety conditions for staff, Italian media reported.
Rome’s transport operator Atac said its “entire network”, including buses, trams and metro lines, would be hit by the walkout.
Passengers face cancellations and delays from 8.30am until 5pm and from 8pm until the end of the service, the provider said.
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People travelling during the fasce di garanzia (‘guaranteed timeslots’) from the start of service until 8.30am and between 5pm and 8pm should not have their journey disrupted by the walkouts.
The following bus routes managed by subcontractors should stay in regular operation throughout the day, ATAC said: 021, 043, 075, 33, 77, 113, 246, 246P, 313, 319, 351, 435, 500, 515, 551, 669, 980.
On the night of Friday December 9th to Saturday December 10th, night bus services (lines starting with ‘n’) will not be guaranteed.
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However, some daytime lines with scheduled services beyond midnight – namely 38, 44, 61, 86, 170, 301, 451, 664, 881 and 916 – should remain in operation.
Local train services, which are operated by national rail company Trenitalia, should not be affected.
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